Digestion of biomolecules in biological matrices results in the formation of thousands of peptides that can interfere with your target molecule. In-silico peptide selection, use of surfactants, immunocapture, micro-elution solid phase extraction and microflow LC-MS/MS (MFLC-MS/MS) with optimal ESI source design were used to improve the selectivity, sensitivity and productivity for the analysis of biomolecules from biological fluids.
About the Presenter
Chad has over 20 years of related experience as an analytical scientist with over 17 years focused on bioanalysis at Alturas Analytics. This depth of experience in applied LC–MS/MS and GC–MS/MS, along with an education in chemical engineering provides the backbone for productive and innovative science. Chad leads the biologics quantitation group at Alturas, applying novel techniques to a regulated, high-throughput production environment. As a Senior Principal Scientist, Chad’s primary focus is LC–MS/MS method development, validation, and sample analysis for small new chemical entities, oligonucleotides, biologics, and antibody drug conjugate (ADC) programs in accordance with GLP guidelines. In addition, Chad leads a team of scientists as Study Director and Principal Investigator, providing technical oversight to clients across all therapeutic areas.