Collection of samples for clinical bioanalysis is typically conducted by a trained phlebotomist at a clinic or hospital and requires a painful blood draw and large volumes of blood. This makes pediatric blood sampling difficult and can be burdensome for the elderly or critically ill. The Tasso-M20 device enables at-home, automated self-collection of blood samples. The device automatically collects 17.5 microliter samples in minutes with very little pain or blood loss. BPC-157 is a pentadecapeptide gastric peptide that possesses free radical scavenging activity and has been shown to reduce inflammation by blocking the production of pro-inflammatory mediators (nitric oxide, prostaglandins, and leukotrienes). BPC-157 has been shown to have healing properties for gastrointestinal fistulas, intestinal lesions, and liver lesions and can be used for the treatment of inflammatory bowel disease and congestive heart failure. BPC-157 has also been shown to accelerate the healing of damaged tendons and ligaments. In order to accurately quantify the peptide in blood collected on the Tasso device an extraction and HPLC-MS/MS method was developed with a dynamic range of 1.00-2000 ng/ml.