Quantitation of MMAE and the Antibody of the Antibody Drug Conjugate Anetumab-MMAE From Rat Plasma Using HPLC-MS/MS and HPLC-HRMS/MS
Authors: Chad David Christianson; Jason Scott Watts; Jennifer S Zimmer; Balaganesh Kuruba
Presented by Chad Christianson at the ASMS 73rd Conference on Mass Spectrometry and Allied Topics
Anetumab-MMAE is an antibody drug conjugate (ADC) composed of a human anti-mesothelin antibody linked to the anti-mitotic cytotoxic agent monomethyl auristatin (MMAE). The ADC targets cancer cells by immune recognition with a receptor-mediated endocytosis cellular MMAE delivery mechanism. Inside the cell, the MMAE binds to tubulins and interrupts the microtubule dynamics resulting in cellular death. Quantitation of ADCs typically require three different measurements using multiple extraction methods and sample aliquots. Free MMAE, conjugated MMAE and total antibody concentrations must be measured and reported. A simple method has been developed in order to quantify all three components. The free and conjugated MMAE was measured using HPLC-MS/MS and the total antibody was quantified using HPLC-HRMS/MS.
Please complete the form below in order to access our resource:
"*" indicates required fields
Manage Consent
To provide the best experiences, we use technologies like cookies to store and/or access device information. Consenting to these technologies will allow us to process data such as browsing behavior or unique IDs on this site. Not consenting or withdrawing consent, may adversely affect certain features and functions.
Functional
Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes.The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
To provide the best experiences, we use technologies like cookies to store and/or access device information. Consenting to these technologies will allow us to process data such as browsing behavior or unique IDs on this site. Not consenting or withdrawing consent, may adversely affect certain features and functions.
Functional
Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes.The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
