Determination of protein binding levels and free circulating drug in biological systems is important in establishing pharmacokinetic activity. The portion of drug that binds to protein within the body is rendered inactive and unavailable to work against the intended target of the drug. In this study, a comparison was performed between a novel sample preparation technique utilizing BioSPME (biocompatible solid phase microextraction) technology and RED (rapid equilibrium dialysis), which is considered the gold standard technique for plasma protein binding level determination. All experimental samples were analyzed via LC-MS/MS. Advantages of the BioSPME technique over current methodologies with respect to sample preparation time and workflow simplification will be presented, as well as a comparison of data accuracy and
About the Presenter
Chad has over 20 years of related experience as an analytical scientist with over 17 years focused on bioanalysis at Alturas Analytics. This depth of experience in applied LC–MS/MS and GC–MS/MS, along with an education in chemical engineering provides the backbone for productive and innovative science. Chad leads the biologics quantitation group at Alturas, applying novel techniques to a regulated, high-throughput production environment. As a Senior Principal Scientist, Chad’s primary focus is LC–MS/MS method development, validation, and sample analysis for small new chemical entities, oligonucleotides, biologics, and antibody drug conjugate (ADC) programs in accordance with GLP guidelines. In addition, Chad leads a team of scientists as Study Director and Principal Investigator, providing technical oversight to clients across all therapeutic areas.